Standard
Phone
Tablet
Desktop
Large Desktop
Very Large Desktop
---

RealStar® PCR Kit optimization for Clostridium difficile and West Nile Virus

 

This product is with immediate effect available as CE IVD:

 

RealStar® Clostridium difficile PCR Kit 2.0 (Ref: 172013) for the detection and differentiation of Clostridium difficile toxin A and toxin B specific DNA.

The RealStar® Clostridium difficile PCR Kit 2.0 was optimized in comparison to RealStar® Clostridium difficile PCR Kit 1.0 to increase the performance of the kit and harmonized within the RealStar® product line.

 

Please note: main change affects the temperature profile and the Internal Control amplification system.

Temperature profile Kit version 1.0: 120sec @95°C, for 45 cycles 15sec @95°C, 45sec @58°C

Temperature profile Kit version 2.0: 120sec @95°C, for 45 cycles 15sec @95°C, 45sec @58°C, 15sec @72°C

For more information Go-to-specifications RealStar® Clostridium difficile PCR Kit 2.0 CE. 

 

 

The following product is available as RUO:

 

RealStar® WNV RT-PCR Kit 2.0 (Ref: 322003) for the detection of West Nile virus specific RNA.

The RealStar® WNV RT-PCR Kit 2.0 was optimized in comparison to RealStar® WNV RT-PCR Kit 1.0 to increase the performance of the kit especially in respect of the Internal Control amplification system.

 

Please note: main changes affect the pipetting scheme and the temperature profile.

  • Reaction volume Kit version 1.0 => 50µL
  • Reaction volume Kit version 2.0  => 30µL
  • Temperature profile Kit version 1.0: 600sec @50°C, 120sec @95°C, for 45 cycles 15sec @95°C, 45sec @55°C, 15sec @72°C.
  • Temperature profile Kit version 2.0: 1200sec @55°C, 120sec @95°C, for 45 cycles 15sec @95°C, 45sec @55°C, 15sec @72°C.

Additionally, for harmonization of the kits from the tropical panel the reference dye ROX was included to the RealStar® WNV RT-PCR Kit 2.0. The CE mark is planned for 2019.

For more information Go-to-specifications RealStar® WNV RT-PCR Kit 2.0 RUO.

 

 

 

Go back