The AltoStar® Automation System AM16 is a robotic pipetting workstation used for sample purification and PCR setup controlled by the AltoStar® Connect software. It is intended to be used in combination with the AltoStar® Purification Kit 1.5, the AltoStar® Internal Control 1.5 and altona Diagnostics PCR Kits for automated purification of nucleic acids and automated assay setup for in vitro diagnostic purposes.
The AltoStar® AM16 is intended for use by professional users trained in molecular biological techniques and the operation of the AltoStar® AM16 instrument.
The AltoStar® Automation System AM16 is a robotic pipetting workstation used for sample purification and PCR setup controlled by the AltoStar® Connect software.
AltoStar® AM16 with description of main elements: The work surface is called a ‘deck’, for placing loadable carriers. These carriers hold reagent containers, such as tubes, microplates, and other kinds of labware.
The UV Kit has been developed in order to offer the possibility of UV decontamination of the AltoStar® AM16 workspace.
Introduction AltoStar® Automation System AM16
AltoStar® Molecular Diagnostic Workflow Video
Key Features
Entire Workflow
The AltoStar® AM16 is CE-IVD marked
DNA and RNA purification in parallel with one-for-all chemistry AltoStar® Purification Kit 1.5 (CE-IVD)
Real-time PCR reaction setup by using the altona Diagnostics PCR Kits (CE-IVD)
Automated cycler programming with AltoStar® Connect software (CE-IVD)
Expandable system for additional thermal cycler (CFX96™ Deep Well Real-Time Detection System-IVD)
Process Safety
Traceability: barcoded reagents for full process control
Ready to use reagents for consistent quality
Lot checking
Expiry date checking
Total Aspiration and Dispense Monitoring (TADM) – proven technology from Hamilton
High Flexibility
All sample types in one run
Analyze up to four pathogens from a single sample
Combine up to eight Real-Time PCR assays on a 96 well plate
Scalable from 1-96 reactions, no minimum batch size
500 µl and 1000 µl sample volume possible in one run
Sample carriers can be unloaded any time after sample processing
Any Sample Type
CE-IVD marked purification kit validated for whole blood, EDTA plasma, urine, stool and swaps in UTM
One purification kit for both DNA and RNA assays
One universal Internal Control for entire workflow (AltoStar® Internal Control 1.5, CE-IVD)
Convenience
Ready to use reagents for extraction and real-time PCR
Use of primary or secondary sample tubes
Freely selectable positions for samples
Load backup reagents and consumables
No refill or mixing of solutions necessary
Full access to eluates
User friendly control software AltoStar® Connect (see under AltoStar® Product Range)
AltoStar® AM16 Automation System
Order No.
AM16
For use with
AltoStar® Purification Kit 1.5
AltoStar® Internal Control 1.5
CFX96™ Deep Well Real-Time PCR Detection System (IVD, Biorad)
Additionally included in package AltoStar® Molecular Diagnostic Workflow
Products
AltoStar® Connect software
AltoStar® Plate Sealer
AltoStar® AM16 PC
Dimensions and Weight
Dimensions (W x D x H)
1124mm x 903mm x 795mm (transport size)
1010mm (with loading tray)
1200mm (with loading tray and carriers on loading tray)
Weight
Approx. 140kg
Operating Data
Maximum Power Consumption
≤ 600VA
Voltage
115 VAC / 230 VAC ± 10%
Delayed Action Fuse
115 V~: 6.3 A (T6.3AL250)
230 V~: 3.15 A (T3.15A250)
Installation Category
II
Pollution Degree
2
Temperature Range
15°C to 30°C / Indoor Use Only
Relative Humidity
15% to 85% (no condensation)
Noise Level
< 65dBA (regarding EN27779)
Altitude
Up to 2000m above sea level
Transportation and Storage
Transportation
Truckage company /AltoStar® AM16 Instrument is
installed or relocated by a Hamilton trained
field service engineer.
Temperature Range
-25°C to +70°C / Indoor Storage Only
Relative Humidity
10% to 90% (no condensation, indoors)
AltoStar® Automation System AM16
AltoStar® Automation System AM16 Operator's Manual IVD
• Input samples and PCR assays assignements - ---> or import all information from LIMS - Create run
• Preparation/pretreatment of samples - Start purification - Load reagents and samples
• Sample purification results - Purification run results are saved in the AltoStar® Connect SW
• Unload reagents and samples after purification
PCR Setup
• Prepare reagents (thawed, mixed, centrifuged) and samples (Eluate plate)
• Prepare material required for the PCR run (PCR Plate Sealing Foil, Plate Sealer, CFX96™ Cycler)
• Start PCR Setup run ---> System now performs the PCR setup automatically
• PCR setup run results are saved in the AltoStar® Connect software
PCR and Analysis
• After a PCR setup run a PCR setup report (pdf file), a file for the LIMS and a cycler file are created
• Cycler file contains information regarding the temperature profile, fluorescence detector settings,
controls (QS and NTC) and sample names - Start PCR run
• PCR results automatically be saved in a defined folder in LIMS and a PDF-report is created at a
predefined location for analysis
AltoStar® Automation System AM16
Declaration of Conformity AltoStar® Automation System AM16
